2019-02-19 20:02:072025-05-30 00:57:11
description
motility regulator, binds to the [[gene|hag ]]mRNA to inhibit its translation
motility regulator, binds to the [[gene|hag]] mRNA to inhibit its translation, promotes the base pairing between the [[gene|sr1]] RNA and the [[gene|ahrC]] mRNA
locus
BSU35370
BSU_35370
product
motility regulator
RNA chaperone, motility regulator
outlinks
bsu
BSU35370
BSU_35370
The protein
Catalyzed reaction/ biological activity
[[protein|CsrA]] binds the ''[[gene|hag]]'' mRNA to inhibit translation [Pubmed|21895793]
[[protein|CsrA]] binds the ''[[gene|hag]]'' mRNA to inhibit translation [Pubmed|21895793]
binds [[gene|sr1]] RNA and [[gene|ahrC]] mRNA to promote their base pairing and to control arginine metabolism [pubmed|31043113]
The protein
Protein family
csrA family (according to Swiss-Prot)
CsrA/RsmA family (single member, according to UniProt)
Biological materials
Mutant
MGNA-A392 (csrA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/392 NBRP B. subtilis, Japan]
GP469 (spc), available in [SW|Stlke] lab
BKE35370 ([[gene|csrA]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE35370 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTCGTTTATTTTCCGCGATA, downstream forward: _UP4_TGAGGATTTTTTTATTTTTG
BKK35370 ([[gene|csrA]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK35370 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTCGTTTATTTTCCGCGATA, downstream forward: _UP4_TGAGGATTTTTTTATTTTTG
MGNA-A392 (csrA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/392 NBRP B. subtilis, Japan]
GP469 (spc), available in [SW|Jörg Stülke]'s lab
BKE35370 ([[gene|csrA]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE35370 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTCGTTTATTTTCCGCGATA, downstream forward: _UP4_TGAGGATTTTTTTATTTTTG
BKK35370 ([[gene|csrA]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK35370 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTCGTTTATTTTCCGCGATA, downstream forward: _UP4_TGAGGATTTTTTTATTTTTG
Biological materials
Expression vectors
pGP381 (N-terminal Strep-tag, purification from ''B. subtilis'', for [SW|SPINE], in [SW|pGP380]), available in [SW|Stlke] lab
for expression, purification in ''E. coli'' with N-terminal His-tag, in [SW|pWH844]: pGP383, available in [SW|Stlke] lab
pGP381 (N-terminal Strep-tag, purification from ''B. subtilis'', for [SW|SPINE], in [SW|pGP380]), available in [SW|Jörg Stülke]'s lab
for expression, purification in ''E. coli'' with N-terminal His-tag, in [SW|pWH844]: pGP383, available in [SW|Jörg Stülke]'s lab
Biological materials
lacZ fusion
pGP461 (in [[protein|pAC7]]), available in [SW|Stlke] lab
pGP461 (in [SW|pAC7]), available in [SW|Jörg Stülke]'s lab
References
Reviews
References
Original Publications
The protein
Paralogous protein(s)
[[this]]
The protein
[SW|Domains]
Contacts FliW via its C-terminus (residues 49-58) (according to UniProt)
The protein
[SW|Localization]
cytoplasm (according to UniProt)